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xPONENT® Software Solutions
FLEXMAP 3D®
Taking the pioneering xMAP® Technology to the next dimension, the FLEXMAP 3D system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to allow multiplexing of up to 500 unique assays within a single sample, both rapidly and precisely.
FLEXMAP 3D Overview
High Plex Features of FLEXMAP 3D Instrument
Features such as automated probe height adjustment, simpler routine maintenance operations and an intuitive software interface make FLEXMAP 3D a flexible and easy to use multiplexing platform capable of performing a variety of protein and nucleic acid applications.
Benefits of FLEXMAP 3D
- Higher Multiplexing = Higher Throughput
Multiplex up to 500 analytes per well. Each microsphere is impregnated with different amounts of three internal dyes. Monitoring the relative intensity of the three signals allows the FLEXMAP 3D system to discriminate up to 500 different microsphere sets. - Enhanced Pipetting = Fast Turnaround Time
The FLEXMAP 3D system has the capability to run 48,000 data points in less than one hour. Dual sample fluidics paths and increased syringe injection speed facilitates a faster sample injection rate. - More Flexibility with 96 and 384 Well Formats
The FLEXMAP 3D accommodates 96 and 384-well formats for enhanced sample volume flexibility and increased throughput. Sample plate heating control also allows the user to run thermally sensitive applications such as nucleic acids. - Automation/LIS Compatibility
xPONENT® 4.0 Software controls the FLEXMAP 3D system and offers interfacing options for Laboratory Information Systems (LIS) as well as automation. An optional swivel base for the system further simplifies integration with third-party liquid handlers. - Simplified Maintenance
The FLEXMAP 3D system has multiple features allowing walk away start-up, shutdown and maintenance operations, and greatly enhances usability for High Throughput Screening (HTS) applications.
The FLEXMAP 3D system is a class 1(I) laser product.
FLEXMAP 3D Demonstration Video
Product Specifications
| Performance | |
| Multiplexing Capability | Up to 500 individual analytes |
| Dynamic Range | 4.5 logs |
| General | |
| Physical Dimensions | 58.4 cm W x 63.5 cm D x 54.7 cm H (23” W x 25.7” D x 18” ) |
| Weight | No more than 200 lbs (91 Kg) |
| Plate Heater Operating Range | 35 – 60ºC (95 - 131ºF) |
| System Warm Up* | 30 minutes |
| Fluidics | |
| Sheath Fluid Pressure | 8 – 15 psi |
| Sample Injection Rate | 2 μl/second |
| Sample Uptake Volume | 20 – 200 μl |
| Optics | |
| Classification Laser | 635 nm |
| Reporter Laser | 532 nm |
| Reporter Detector | 565nm – 585nm |
* The FLEXMAP 3D system is a class 1(I) laser product.
xPONENT® Software Solutions
Data Acquisition and Analysis for Luminex Instrumentation
xPONENT is a modular, flexible, software package for control of the MAGPIX®, Luminex® 100/200™ and FLEXMAP 3D. The software’s graphical user interface follows the typical assay workflow but can be customized to meet the needs of a specific laboratory.
Software Configurations
xPONENT software is available with three levels of accessibility.
Basic
The basic xPONENT software package allows the user to acquire samples, perform analysis, and run system maintenance routines and remote web monitoring. This package includes the ability to calculate allelic ratios to analyze and/or normalize genotyping data.
Secure
The secure xPONENT package includes all the basic functionality, plus allows an administrator to set up user access levels.
21 CFR Part 11
For laboratories working under the FDA’s 21 CFR Part 11 regulations, xPONENT offers multilevel user management, full audit trail, electronic records and electronic signatures.
In addition to the access levels listed above, there are two additional licensable modules that add specific functions:
Automation Connectivity
The automation module along with a device driver enables communication between the reader and a front end automation platform. Luminex device drivers are offered by many automation providers. For more information on available drivers please contact Luminex Technical Support.
LIS Connectivity
This module allows communication with an external Laboratory Information System (LIS) database by allowing the export of patient result data and the import of patient sample information in ASTM format. Connection to your LIS system may require the use of middleware. For more information on middleware solutions please contact Luminex Technical Support.
Ordering Information
| Luminex Direct | Luminex Partners |
|---|---|
| Luminex Online Order Management Email: orders@luminexcorp.com Phone: 1-512-336-3550 Toll Free: 1-866-401-5450 Fax: 1-512-219-0544 Fax: 31-162-408330 (EMEA, India) |
Bio-Rad Laboratories EMD Millipore Corporation Life Technologies |
| Product Description | Luminex Part Number | List Price USD | Description |
|---|---|---|---|
| Instrument | |||
| FLEXMAP 3D | Order Information | Versatile multiplexing platform | |
| Reagents | |||
| FLEXMAP 3D Calibration Kit | FM3D-CAL-K25 | Order Information | xMAP® Calibrators for MagPlex® and MicroPlex® Classification Channels and Calibrator for Reporter Channel |
| FLEXMAP 3D Performance Verification Kit | FM3D-PVER-K25 | Order Information | xMAP Verification Reagents to Verify Calibration of MagPlex and MicroPlex Classification Channels and Reporter Channel |
| Sheath Fluid | 40-75680 | Order Information | Sheath Concentrate Pack |
| 40-50000 | Order Information | 20 L Sheath Fluid | |
| Accessories | |||
| Sample Probe Needle | CN-0221-01 | $76.00 | Stainless steel sampling needle for use with the FLEXMAP 3D system |
| Sample Probe Height Adujustment Kit | CN-0263-01 | $27.00 | Probe height adjustment tools for use with the FLEXMAP 3D system |
| FLEXMAP 3D Off-Plate Reagent Block | CN-0225-01 | $89.00 | Calibration, Performance Verification and Maintenance plate for performance testing of the FLEXMAP 3D system |
| Heater Block, 96-Well Microtiter Plate | CN-0224-01 | $76.00 | Anodized aluminum heating block compatible with 96-well V-bottom plates for use on the FLEXMAP 3D system |
| Heater Block, 384-Well Microtiter Plate | CN-0223-01 | $109.00 | Anodized aluminum heating block compatible with 384-well V-bottom plates for use on the FLEXMAP 3D system |
| Bar Code Scanner | CN-PC03-01 | $841.00 | Handheld USB Bar Code Scanner for use with the FLEXMAP 3D instrument |
| FLEXMAP 3D Swivel Base | CN-0216-01 | $4,116.00 | Optional swivel base that allows the user to rotate the instrument for ease of maintenance. Swivel base is commonly used when integrating to front-end liquid handlers. |
| Accessories | |||
| Syringe, 500 mL with Seal | CN-0013-01 | $243.75 | Replacement syringe assembly for the FLEXMAP3D system |
| Syringe seal, (qty 4) | CN-0014-01 | $118.75 | Replacement seals for FLEXMAP 3D 500 mL syringe |
| 6-Month High Throughput PM Kit | CN-0215-01 | $53.00 | Preventive maintenance kit for high throughput applications. Includes HEPA air filter and 500 mL syringe seal |
| Fuse (6 AMP) | CN-0226-01 | $22.00 | Replacement fuse located inside the power module. |
| Cable USB (A to B) | CN-0227-01 | $22.00 | Communications cable between FLEXMAP 3D instrument and PC. |
| Air Filter HEPA | CN-0228-01 | $12.00 | Replacement high efficiency air filter located behind the panel on the left side of the fluidics bay. |
| Air Filter, XY Platform | CN-0229-01 | $32.00 | Replacement air filter located on the front panel of the XY module |
| Air Filter: Access Door | CN-0222-01 | $29.00 | Replacement front door ventilation filters |
Downloads
| Document | Language | Type | Download |
|---|---|---|---|
| Manuals and MSDS |
|||
| FLEXMAP 3D Hardware User Manual | English | pdf  |
 |
| Manuals |
|||
| MSDS | |||
| Data Sheets and Brochures | |||
| FLEXMAP 3D Overview | English | pdf |
|
| FLEXMAP 3D Brochure | English | pdf |
|
| FLEXMAP 3D High Density Screening Tech Sheet | English | pdf |
|
| FLEXMAP 3D High Throughput Tech Sheet | English | pdf |
|
| FLEXMAP 3D Hardware User Manual | English | pdf |
|
| Luminex Instrument Comparison | English | pdf |
|
| xMAP Technology Analyte Menu | English | pdf |
|
| xMAP Technical Sheet | English | pdf |
|
| FLEXMAP 3D Automation Data Sheet | English | pdf |
|
FLEXMAP 3D Training Courses
| Course Title | Format | Duration |
|---|---|---|
| FLEXMAP 3D & xPONENT® | Online | 1 hour |
| FLEXMAP 3D & xPONENT Basic | Classroom | 1 day |
Support Information
Luminex offers comprehensive technical support enabling companies and laboratories to perform bioassays quickly, accurately, and cost effectively while optimizing the customer experience with Luminex systems.
| Support Resources | Technical Support | Field Service | ||
|---|---|---|---|---|
| Protocols | Online Support Login | Certified Service Providers | ||
| Technical Tips | Email: support@luminexcorp.com | Maintenance and Support | ||
| Target Values | Phone: 512-381-4397 | Luminex Central Repair Facility | ||
| Recommended Materials |
Toll Free: 1-877-785-2323 | |||
| International: + 800-2939-4959 |
PC Warranty Transfer Information
Dell provides a limited warranty with PCs shipped from Luminex. Upon receipt of the Dell PC that is associated with your Luminex System, please complete the warranty transfer process. This will ensure the newly acquired PC is in your name and that you will receive technical support from Dell for the remainder of your warranty period. Required Items: Service Tag Number and Express Service Code (ESC) – located directly on the Dell PC.
